Featured Reversed Phase HPLC Columns

Luna Omega Polar Columns

Luna Omega Polar C18 UHPLC Columns

  • 100% Aqueous Stable
  • Enhanced Selectivity for Polar Compounds
  • Unique retention for Non-polar Compounds
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EVO C18 Reversed Phase Column

Kinetex EVO Core-shell
C18 LC Column

  • Greater Polar Retention with 100% Aqueous Stability
  • Robust Methods from pH 1-12
  • Improved Peak Shape for Bases
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Luna Omega Polar Columns

Luna Omega C18 UHPLC Columns

  • Astounding UHPLC performance and efficiencies
  • Better peak shape through an inert foundation
  • Extreme ruggedness and dependability
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Simple Reversed Phase LC Method Development

Develop Reversed Phase Methods in Minutes

Separate Hydrophobic Compounds with Reversed Phase LC

A Quick Look at Reversed Phase HPLC

Being the most common principle HPLC/UHPLC separation mode, reversed phase chromatography offers dynamic retention of compounds with hydrophobic and organic functionality. Retention of these compounds by reversed phase involves a combination of hydrophobic and van der Waals type interactions between each target compound and both the stationary phase and mobile phase.


Stationary phases used in reversed phase chromatography typically consist of varying lengths of hydrocarbons such as C18, C8, and C4 or strongly hydrophobic polymers such as styrene divinylbenzene.

  • C18 HPLC columns are the most preferred as they offer an excellent range of hydrophobic separation power along with high surface area coverage
  • C8 HPLC columns are used when less retention compared to a C18 is needed
  • C4 and C5 HPLC columns are commonly used to separate large macromolecules such as proteins
Reverse phase chromatography molecule

Featured Normal Phase HPLC Columns

Luna Silica HPLC Phase

Luna HPLC Columns

  • Luna Silica HPLC columns
    recommended for L3 USP methods
  • Luna CN HPLC columns
    recommended for L10 USP methods
  • Luna Amino HPLC columns
    recommended for L8 USP Method
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Separate Compounds with Normal Phase LC

A Quick Look at Normal Phase

Normal phase chromatography (NPC) is used to separate hydrophobic compounds and matrices that are retained too strongly by reversed phase and have minimal solubility in aqueous mobile phases.


Normal phase stationary phases typically include polar functional groups (silica, amino, and cyano) and interact with analytes primarily via dipole-dipole and hydrogen bonding interactions.

Silica HPLC Column Stationary Phase CN HPLC Column Stationary Phase NH2 HPLC Column Stationary Phase

Featured HILIC Phase HPLC Column

Kinetex HILIC HPLC Phase

Kinetex HILIC HPLC Columns

  • Novel core-shell unbonded silica phase for UHPLC/HPLC separations
  • Provides significant selectivity for retention and separation of polar compounds
  • pH stable from 2-7.5
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Separate Polar Compounds with HILIC LC

A Quick Look at HILIC

HILIC is a particularly useful separation mode for polar organic compounds that are poorly retained by reversed phase. Retention of these polar compounds using reversed phase methods is often difficult because of co-elutions with the solvent front or elutions within the chromatographic region where ion suppression is the greatest.


HILIC HPLC/UHPLC columns draw and retain a water-enriched layer onto the surface of the silica which facilitates the interaction of polar compounds with the stationary phase for increased retention.

Featured Ion Exchange HPLC Column

HPLC Columns

Luna SCX HPLC Columns

  • Ultra pure silica
  • Strong selectivity for positively charged compounds
  • Available in 5 and 10 µm particle sizes
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Retain Positively Charge Analytes with Ion Exchange

A Quick Look at Ion Exchange

Ion-exchange (IEX) chromatography involves interactions between a charged stationary phase and the oppositely charged mobile analytes. In cation-exchange chromatography positively charged molecules are attracted to a negatively charged stationary phase. Likewise, in anion-exchange chromatography negatively charged molecules are attracted to a positively charged stationary phase.


Ion-exchange (IEX) chromatography is useful for a wide variety of compounds, such as acidic (anionic) and basic (cationic) small molecules up to peptides and proteins.

Ion Exchange HPLC Column Stationary Phase Benzene Sulfonic Acid

Featured Ion Exclusion HPLC Column

Ion Exclusion HPLC Columns

Rezex Ion Exclusion HPLC Columns

  • Excellent resolution for carbohydrates and organic acids
  • USP L17, L19, L22, L34, and L58 packings available
  • Multiple ionic forms for a wide range of selectivities
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Separate Organic Acids with Ion Exclusion

A Quick Look at Ion Exclusion

Ion exclusion (IEC) chromatography is a process of separating components in a mixture by means of an ion-exchange resin that excludes highly ionized particles and retains slightly ionized or non-ionized particles. Ion exclusion (IEC) chromatography applies to the retention of organic acids, carbohydrates, sugars, starches, and oligosaccharides, using a sulfonated stationary phase.


From drug formulation and excipient analysis to quality control testing of finished food products to fermentation monitoring of bioethanol production, ion exclusion provides the necessary accurate and reproducible analytical results.

Mannitol can be retrained using Ion Exclusion HPLC Columns